D.J. Qualls was born in Nashville, TN and has 4 siblings. He studied at the University of London and returned to Tennessee where he started acting in the local theatre. He was discovered by photographers David La Chappelle and Steve Klein, and he soon began modeling for Prada. His first role was in the film Road Trip and he appeared in Scrubs soon after. He can also be seen in episodes of Lost, CSI, Monk, and My Name is Earl. DJ has also starred in the film The New Guy and has appeared in the films Little Athens, Delta Farce, and The Core.
Qualls is a cancer survivor and an advocate for cancer research and awareness.